Test Name

Bilirubin
Direct

Test Code

230

Unit

mg/dL

Station

Chemistry

Synonym Name/Abbreviation

Conjugated Bilirubin

CPT Code

82248

Performing Facility

American Medical Labs, Herzliya
Pituah

Analyzer

Roche cobas 6000

Test Method

Colorimetric
(Jendrassik-Grof)

AML Preferred Tube

5 mL SST gel

Storage Instructions

Refrigerated.

Separate serum from cells within 45 minutes.

Protect
from light.

Stability:

2 days at 15 to 25 ^oC.

7 days at 2 to 8 ^oC.

6 months at -25 to -15 ^oC.

Min. Volume

1 mL

Turn-Around-Time

One day.

Intended Use

Evaluate liver and biliary disease. Increased direct
bilirubin occurs with biliary diseases, including both intrahepatic and
extrahepatic lesions. Hepatocellular causes of elevation include
hepatitis, cirrhosis, and advanced neoplastic states. Increased with
cholestatic drug reactions, Dubin-Johnson and Rotor
syndromes.

Limitations

Measurement
of direct bilirubin is usually not necessary when the total bilirubin is
<1.2 mg/dL

Measuring range: 0.09 to 17
mg/dL.

Patient Preparation

No preparation needed

Reasons for Rejection

Gross hemolysis, gross lipemia, specimen not protected from
light, improper labeling.

Adult Reference Ranges

0.0 – 0.3
mg/dL